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For the studies with mature OLs, cells were directly incubated in SATO medium supplemented with 0.5% fetal calf serum (FCS) and cultured for 7 days (Bodinco, Alkmaar, the Netherlands)

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For ferrets, rabbits, guinea pigs, and other small pets.

doi: 10.1016/j.freeradbiomed.2010.07.027

If youre wondering how to reuse your nickel agarose beads, the great news is that its really easy to clean them up and prepare them for another round of purification

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